Total Acetyl-CoA Carboxylase Sandwich ELISA Kit

Total Acetyl-CoA Carboxylase Sandwich ELISA Kit

Cat. No.: DAA-001020

Size: 1 x 96 tests Size: 10 x 96 tests Size: Customer Size
Product Information
Product Name Total Acetyl-CoA Carboxylase Sandwich ELISA Kit
Species Human
Description The Total Acetyl-CoA Carboxylase (ACC) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ACC. An ACC mouse antibody has been coated onto the microwells. After incubation with cell lysates, ACC (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, an ACC rabbit detection antibody is added to detect captured ACC protein. Anti-rabbit IgG, HRP-linked AntibodyIt is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total ACC.
Component Acetyl-CoA Carboxylase Mouse mAb Coated Microwells; Acetyl-CoA Carboxylase 1 Rabbit Detection Ab; Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated); Detection Antibody Diluent; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Sample Diluent; Sandwich ELISA Lysis Buffer (1X)
Target Information
Target Name ACACA; ACACB
UniProt No. Q13085; O00763
Target Description Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA. It is the key enzyme in the biosynthesis and oxidation of fatty acids. In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues. However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs.
Alternative Names Acetyl-CoA carboxylase 1; Acetyl-CoA carboxylase 2
Shipping & Storage
Sipping Shipping on dry ice.
Storage Store at -20°C. Please refer to protocols.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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