Total Akt1 Sandwich ELISA Antibody Pair

Total Akt1 Sandwich ELISA Antibody Pair

Cat. No.: DAA-001045

Size: 1 x 96 tests Size: 10 x 96 tests Size: Customer Size
Product Information
Product Name Total Akt1 Sandwich ELISA Antibody Pair
Species Human; Mouse
Description Total Akt1 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our Total Akt1 Sandwich ELISA Kit. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Akt Rabbit Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by Akt1 Mouse Detection Antibody and HRP-conjugated Anti-Mouse IgG. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Total Akt1 protein. Antibodies in kit are custom formulations specific to kit.
Component Akt Capture Rabbit mAb (100X); Akt1 Detection Mouse mAb (100X); Anti-mouse IgG, HRP-linked Antibody (1000X)
Target Information
Target Name AKT1
UniProt No. P31749
Gene ID 207
Target Description Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin. LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insulin stimulation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin.
Alternative Names Protein kinase B (PKB); Protein kinase B alpha (PKB alpha); Proto-oncogene c-Akt; RAC-PK-alpha
Shipping & Storage
Sipping Shipping on dry ice.
Storage Store at -20°C. Please refer to protocols.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
Related Products
logo
Contact Info
Copyright © Ace Metabolism. All Rights Reserved.
Top